2.8. TCBS selectiÕity
Strains were isolated on TCBS Difco brand . A representative sample of isolates thatŽ . did not correspond to the basic Vibrio spp. taxonomic characteristics were then seeded on Oxoid, Merk and Eiken TCBS agar media and their growth percentages were recorded. In this way, we determined whether the bacterial population could grow in any TCBS media or if there was a problem of media efficiency in Artemia cysts samples.
2.9. Statistical analysis
Correlations of cysts age and percentage of hatched cysts were accomplished with an statistical program for PC computers Stat Soft, 1993 . Dendrogram was obtained with aŽ . 1-Pearson r similarity matrix and grouped with the unweighted average linkage method ŽSneath and Sokal, 1973 ..