For shoot multiplication, the data was recorded for different
parameters including shoot initiation percentage (number of explants
initiating shoots were counted and percentage was calculated),
average number of shoots per explant (number of shoots
initiated, of five explants per replication, were counted and average
was calculated), average shoot length (cm) (shoot length of five
explants per replication, were counted and average was calculated),
average number of leaves per shoot (number of leaves of
five explants per replication, were counted and average was
calculated) and average number of nodes per shoot (number of
nodes per shoots were noted for five explants per replication and
average was calculated). Shoot initiation percentage was calculated
after one week, whereas all the other parameters were taken after
eight weeks interval.