The tubes were vortexed and allowed to lyse for 30 min at 4 C. The tubes were centrifuged for 15 min at 15,000 g (4 C) and the supernatant was transferred to a labeled conical glass tube.
The tubes were vortexedand allowed to lyse for 30 min at 4 C. The tubes were centrifugedfor 15 min at 15,000 g (4 C) and the supernatant wastransferred to a labeled conical glass tube.