Several methods have been employed to synthesize silver nanoparticles among which, the chemicalreduction method has been extensively investigated because of its simplicity of performance and mild conditions.
Usually, the chemical reduction is composed of silver nitrate as a precursor, reducing agent, and stabilizer. The stabilizing agents are used to separate the particles to prevent aggregation.
Moreover, phospholipids as a stabilizer are used to improve the biocompatibility of synthesized metal nanoparticles . Park et al reported the membrane fluidity of loaded nanoparticles to the liposome, while the synthesis of nanoparticles in phospholipid membrane had been reported already. In this study, the effect of phospholipid concentrations on the antibacterial and killingkinetic of two Gram-positive and Gram-negative bacteria was studied and the Chick-Watson model was adopted for evaluation of AgNPs disinfection kinetics.