The amino acid is the added in spots to their corresponding origins.
This is done using a capillary tube. Try to keep the spots as small as
possible. You may want to practice the spotting of a solution on
paper. Practice with water and a paper towel (or a piece of filter
paper). Do not waste the chromatographic paper for practicing.What you
want is a small spot of solution on the paper--about 2mm (.2 cm in
diameter). If you want more material put on the paper, you should wait
until the first spot has dried before applying a second spot of the
same material. All in all about six spots are sufficient. To speed up
time a hair dryer can be used to dry the amino acids quicker. Do not
use the same capillary for each--you will contaminate each spot with
the previous substance. Use a different capillary for each solution