4. Conclusions
A reliable construct- and event-specific real-time PCR detection
method for 55-1 was developed. Although the quality of the puri-
fied DNA samples varied greatly among products, our method was
found to be useful for detecting 55-1 in fresh fruit and eight types
of processed products containing papaya as a main ingredient.
Monitoring papaya products for the presence of GM papaya was
demonstrated using a P35S and T-nos real-time PCR detection
method. A collaborative variability test using this technique is currently
underway.
Acknowledgements
We thank Yoshihiro Ozeki, Satoshi Futo, and Hiroyuki Haraguchi
for their useful suggestions. This study was supported by a
grant from the Ministry of Health, Labor, and Welfare of Japan.
Appendix A. Supplementary data
Supplementary data associated with this article can be found, in
the online version, at http://dx.doi.org/10.1016/j.foodchem.2012.
08.088.