The first protocol is an example on how to incorporate molecules of interest into silk microspheres using DOPC as an emulsifier. The average particle size of spheres made with this method is approximately 2μm, which can be determined with either optical microscopy or SEM54. As for drug loading, different drugs will interact differently with the silk and therefore the loading efficiency will differ between various molecules. We suggest that small batches are tested in the process before scaling up. Resuspended microspheres can also be mixed with silk solution and then be made into porous scaffolds, hydrogels or films.