Detection of Antioxidant activity on TLC plate- The samples were
applied on a 2.5 mm silica gel 60 F254 TLC plate. After the plate had been
developed, it was dried at room temperature and then sprayed with 0.2 mM DPPH in
methanol. The DPPH solution was freshly prepared and stored in darkness. After the
plate was dried at room temperature, an active spot indicating antioxidant was a
colorless spot on the purple background.