Two column packings – 15% SP-1220/1% H3
PO4
on Chromosorb
W AW and 10% SP-1000/1% H3
PO4 on Chromosorb W AW –
provide excellent resolution of VFAs and nonvolatile acid methyl
esters in fermentation products. For greater accuracy with difficult
samples, use both packings to confirm results. The SP-1220
packing offers more rapid separations, while the SP-1000 packing
provides better separations of the nonvolatile acid methyl esters
and isolates the acids from short chain alcohols which may be
present. (To isolate lactic acid from fermentation products, see
the next section of this bulletin.