MIC2 is discharged at the parasite apex and is progressively capped during the invasion of host cells. Four separate examples are depicted to illustrate attachment to the cell surface (®rst row), partial penetration (second row), nearly completed invasion (third row) and completed invasion (fourth row). Cells were semi-permeabilized to reveal the contents of the parasitophorous vacuole, which is delineated by ROP1. In the ®rst row, MIC2 is found on the apical surface (arrowhead) of a parasite that is bound to the host cell but not yet invaded (no ROP1 release). In the second row, MIC2 is restricted to the extracellular portion of the parasite that lies distal to the moving junction (arrowhead), while ROP1 is released from the anterior end and ®lls the parasitophorous vacuole. In the third row, MIC2 is capped to the posterior end of the parasite (arrowhead) before being shed prior to completion of entry (fourth row).
MIC2 is discharged at the parasite apex and is progressively capped during the invasion of host cells. Four separate examples are depicted to illustrate attachment to the cell surface (®rst row), partial penetration (second row), nearly completed invasion (third row) and completed invasion (fourth row). Cells were semi-permeabilized to reveal the contents of the parasitophorous vacuole, which is delineated by ROP1. In the ®rst row, MIC2 is found on the apical surface (arrowhead) of a parasite that is bound to the host cell but not yet invaded (no ROP1 release). In the second row, MIC2 is restricted to the extracellular portion of the parasite that lies distal to the moving junction (arrowhead), while ROP1 is released from the anterior end and ®lls the parasitophorous vacuole. In the third row, MIC2 is capped to the posterior end of the parasite (arrowhead) before being shed prior to completion of entry (fourth row).
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