The yeast strains showing fat globules within the
cell were selected for further quantitative analysis. The yeast strains were precultured in inoculum
medium, and then 24 h old preculture were inoculated in 25 mL crude glycerol based (crude glycerol 100
g/L with corresponding glycerol concentration of 40 g/L, yeast extract 10 g/L and peptone 10 g/L, pH
6.0) at the C/N molar ratio of 7.4 and incubated for 72 h at room temperature with shaking at 140 rpm.