Extraction of plant materials
Plants collected from various families were brought to the laboratory, washed with dechlorinated water,
shade dried under room temperature and the plant materials were powdered individually using an
electric blender. Each powdered plant material were sieved using a kitchen strainer. One kilogram of each
powdered plant material was sequentially extracted with hexane, diethyl ether, dichloromethane and
ethyl acetate for a period of seventy two hours and then filtered. The filtered content was then subjected
to rotary vacuum evaporator until solvents were completely evaporated to get the solidified crude
extracts. The crude extracts thus obtained were stored in sterilized amber coloured bottles maintained at
40C in a refrigerator. Standard one per cent stock solution was prepared by dissolving 100 mg of crude
extract in 100 ml of acetone.