The lytic activity of vibriophages (ATCC B1, B2, B3, B4, and B5) and the cocktail were evaluated in in vitro. V. cholerae O1 MAK 757 was mixed with the phages or the phage cocktail separately at an MOI of 0.1 and incubated at 37 °C with shaking. Results showed that the phage cocktail was more potent as a lytic agent than the individual phagesant.