2.3. Statistical analysis
The four treatments were distributed in a completely randomized design with three replicates.
Data were subjected to the analysis of variance test (ANOVA) using the SUPERANOVA computer package (Abacus Concepts Inc, Berkeley, CA, USA; 1998).
A separation test on treatment means was conducted using Fisher’s least significant differences (LSD) methods at 95% confidence level (Snedecor & Cochran, 1988).