Separation methods based on cloud point extraction (CPE) are
practical application of surfactants in analytical chemistry and
have become an alternative to solvent extraction. Compared with
conventional solvent extraction, CPE uses surfactants and avoids
utilizing a large amount of expensive, toxic, and flammable organic
solvents. CPE procedure has been widely used for separation,
purification and pre-concentration of variety of substances such
as organic and inorganic compounds in water, food, drug and
biological samples [15–24].