This case does not quite meet the criteria defined currently in the OIE WTD disease card, mainly due to 179 Primer set Location Accession no. Description Identity (%) Sri Widada et al. French West Indies AY222840.1 Macrobrachium rosenbergiinodavirus segment 91.04 (2003) RNA-2, complete sequence China AY231437.2 Macrobrachium rosenbergiinodavirus capsid 90.89 protein gene, complete coding sequence Yoganandhan et al. India DQ146969.1 Macrobrachium rosenbergiinodavirus RNA- 95.92 (2006) directed RNA polymerase gene, partial coding sequence French West Indies AY222839.1 Macrobrachium rosenbergiinodavirus segment 95.58 RNA-1, complete sequence Sri Widada et al. Thailand EU150133.1 Macrobrachium rosenbergiiXSV isolate M23 99.38 (2004) capsid protein gene, complete coding sequence Thailand EU150132.1 Macrobrachium rosenbergiiXSV isolate M308 99.38
capsid protein gene, complete coding sequence Table 1. Top 2 alignments giving the highest scores for sequence identity between Australian Macrobrachium rosenbergii nodavirus (MrNV) and other geographical isolates Fig. 7. Phylograms of the sequence the Australian isolate of Macrobrachium rosenbergiinodavirus (MrNV) compared to other isolates of MrNV using both sequences from RNA-1 (a) 861 nt and RNA-2 (b) 692 nt. Phylograms were produced by ClustalW2
Dis Aquat Org 85: 175–180, 2009 the age of the prawns involved. Most previous reports have involved postlarvae or very young juveniles, whereas this case involved adults and older juveniles. The gross signs, the histopathological lesions in muscle and the confirmatory genome sequence are all consis-tent with the OIE definitions. The mortality in experi-mental prawns was chronic and limited over the time frame of the study, and inclusion bodies were not seen
in connective tissue but rather in the muscle, staining
more like RNA (pyroninophilic). Based on the findings
in this case, perhaps the OIE definition needs to be
broadened somewhat to include adult life stages, and
the staining characteristics should be reevaluated