Glycosylated haemoglobin was determined according to the ion exchange resin method .Blood urea was estimated
by urea- glutamate dehydrogenase (GLDU) method.
Estimation of lipid profile
Total cholesterol estimation was done using the Erba diagnostic kit Serum triglyceride were estimated using
Enzokit (Ranbaxy) .HDL cholesterol was determined using the Erba diagnostic kit. VLDLcholesterol was
calculated as Triglyceride/5.