Culture volume and agitation speed affectedthe dissolved oxygen profile of B. bassiana cultures as well astheir growth parameters. Higher aeration rates were achievedby lowering culture volume, increasing agitation speed and bytheir combination, resulting in extended oxygen availability asnoted by the dissolved oxygen curves (Fig. 1). Apart from theB. bassiana isolates, the fermentation time where culturesreached 50% dissolved oxygen (DO50) were 4.5–7.3 h higherin cultures grown at 50 mL compared with cultures grown at100mL, and 6.8–9.6 h in cultures grown at 350 rpmcomparedwith cultures grown at 175 rpm (Table S1 in the Electronicsupplementary material; Fig. 1). Furthermore, B. bassianacultures of both isolates showed similar DO50 values. Dissolvedoxygen levels remained low (<10 % oxygen) after24 h growth and throughout the remaining 3 days of fermentationwhen B. bassiana cultures were incubated at 350 rpm,suggesting that these conditions promoted maximum oxygenconsumption
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