However, the number of applications in feed matrices is quite limited, where a group of pesticides, VDs, mycotoxins and plant toxins are determined in horse feed [28, 33] and coccidiostats in animal feed [34]. Therefore, the aim of this work is the development of a reliable and sensitive methodology based on the use of advanced UHPLC–QqTOF–MS technique for the quantitative determination and reliable identification of more than 250 pesticides and VDs in different types of feed matrices using generic methods.