Advantages and Disadvantages
Advantages
• Have an excellent sensitivity comparable to EIA and RIA.
• Reagents are stable and relatively nontoxic.
• The sensitivity of some assays has been reported to be in the
range of attamoles (10-18) to zeptomoles ( 10-21).
• Because very little reagent is used, they are inexpensive to
perform.
• Detection systems basically consist of photomultiplier tubes
which are simple and relatively inexpensive
Disadvantages
• False results may be obtained if there is lack of precision in
injection of the hydrogen peroxide
• If some biological materials such as urine or plasma cause diminishing of the light emission.