2.3. Ethanol and potassium sorbate toxicity to B.
cinerea spores
B. cinerea spores (10,000 spores/ml) were mixed
with various ethanol concentrations, either alone or
in combination with 0.5 or 1.0% potassium sorbate at
O.A. Karabulut et al. / Postharvest Biology and Technology 37 (2005) 129–134 131
ambient temperature (22–24 ◦C) in a final volume of
2 ml. The pH of 0.5 and 1.0% potassium sorbate solutions
was 9.1 and 9.3, respectively. Addition of 10 and
20% ethanol either to 0.5 or 1.0% potassium sorbate
did not change the initial pH of solutions. After 30 s,
the spore suspensions were diluted 100-fold in sterile
water and 100 l were plated on PDA. After 48 h incubation
at 24 ◦C, the colonies per plate were counted.
Data were expressed as the percentage of germinated
spores. The experiment was performed twice.