Additional statistics were computed to estimate the grade of
polymorphism among the studied isolates. The number of amplified loci (n), the maximum (Max) and minimum (Min) band size,
the percentage of polymorphic loci (Ppl), the average of observed
(na) and effective (ne) alleles, the Nei’s gene diversity (h)[32]and
the Shannon information index (I) [27] were calculated for each
primer and among all primers. The discriminant power of each
primer was calculated as the sum of the Gregorius differentiation
coefficients (Sd)[19]of each locus. Average and standard deviation
of all the considered parameters were calculated for ISSR and SRAP
primers, separately, and both together. All calculations and analyses
were conducted using POPGENE, version 1.31
Additional statistics were computed to estimate the grade ofpolymorphism among the studied isolates. The number of amplified loci (n), the maximum (Max) and minimum (Min) band size,the percentage of polymorphic loci (Ppl), the average of observed(na) and effective (ne) alleles, the Nei’s gene diversity (h)[32]andthe Shannon information index (I) [27] were calculated for eachprimer and among all primers. The discriminant power of eachprimer was calculated as the sum of the Gregorius differentiationcoefficients (Sd)[19]of each locus. Average and standard deviationof all the considered parameters were calculated for ISSR and SRAPprimers, separately, and both together. All calculations and analyseswere conducted using POPGENE, version 1.31
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