and a spontaneous bombarded norflurazon resistant strain (bottom 4 rows) and pPlat–pds (first upper row). A. The mutation site conferring norflurazon resistance is marked by a red box (WT: TG; mutant: GC). B. Successful integration of mutated pds into the genome is indicated by polymorphism in pds intron. Various polymorphisms between the BGU strain (samples 13 and 19) and the mutated pds gene used for transformation [37] are marked with black frames on the lower panel. A spontaneously resistant colony (no. 19) does not contain the mutation; resistance is probably due to a different mechanism.