Egg storage longer than 7 d is associated
with a delay in hatch time and a decline in hatchability
and chick quality. Prestorage incubation is suggested
as a method to reduce the negative effects of
prolonged storage times by altering the developmental
stage of the embryo, but earlier research has shown
that prestorage incubation can both be detrimental and
beneficial for hatchability. The reason for these ambiguous
results is not clear and the effect of prestorage incubation
on chick quality is not studied extensively.
The objective of this study was to investigate changes
in developmental stage of embryos during prestorage
incubation and the effect of prestorage incubation on
hatchability and chick quality. Two experiments were
conducted. In experiment I, eggs were stored for 3, 5,
8, or 12 d. In experiment II, eggs were stored for 5 or
11 d. Half of the eggs was stored immediately at 16
to 18°C and the other half was exposed to prestorage
incubation for 6 h in experiment I and for 4.5 h in
experiment II. According to the classification table of
Eyal-Giladi and Kochav (EG), embryonic development
was advanced by prestorage incubation from developmental
stage EG11.67 to developmental stage EG13.26
in experiment I (P = 0.02) and from developmental
stage EG9.22 to developmental stage EG12.63 in experiment
II (P < 0.0001). In experiment I, prestorage
incubation reduced hatchability of set eggs from 59.3 to
51.5% when storage time was 12 d but did not reduce
hatchability when storage time was 3, 5, or 8 d (interaction
P = 0.02). Prestorage incubation increased chick
length (P = 0.004). In experiment II, prestorage incubation
increased hatchability of fertile eggs from 80.6 to
85.9% when storage time was 11 d but did not increase
hatchability when storage time was 5 d (interaction P
= 0.0009). Prestorage incubation increased percentage
of second grade chicks (P = 0.0007). It seems that storage
time, embryonic development at egg collection, and
prestorage incubation duration determine the effect of
prestorage incubation on hatchability and chick quality.
Egg storage longer than 7 d is associatedwith a delay in hatch time and a decline in hatchabilityand chick quality. Prestorage incubation is suggestedas a method to reduce the negative effects ofprolonged storage times by altering the developmentalstage of the embryo, but earlier research has shownthat prestorage incubation can both be detrimental andbeneficial for hatchability. The reason for these ambiguousresults is not clear and the effect of prestorage incubationon chick quality is not studied extensively.The objective of this study was to investigate changesin developmental stage of embryos during prestorageincubation and the effect of prestorage incubation onhatchability and chick quality. Two experiments wereconducted. In experiment I, eggs were stored for 3, 5,8, or 12 d. In experiment II, eggs were stored for 5 or11 d. Half of the eggs was stored immediately at 16to 18°C and the other half was exposed to prestorageincubation for 6 h in experiment I and for 4.5 h inexperiment II. According to the classification table ofEyal-Giladi and Kochav (EG), embryonic developmentwas advanced by prestorage incubation from developmentalstage EG11.67 to developmental stage EG13.26in experiment I (P = 0.02) and from developmentalstage EG9.22 to developmental stage EG12.63 in experimentII (P < 0.0001). In experiment I, prestorageincubation reduced hatchability of set eggs from 59.3 to51.5% เมื่อเก็บ 12 d แต่ไม่ลดhatchability เมื่อเวลาเก็บ 3, 5 หรือ 8 d (โต้ตอบP = 0.02) คณะทันตแพทยศาสตร์ prestorage เจี๊ยบเพิ่มขึ้นความยาว (P = 0.004) ในการทดลอง II, prestorage คณะทันตแพทยศาสตร์เพิ่มขึ้น hatchability ของไข่อุดมสมบูรณ์จาก 80.6 เพื่อ85.9% เมื่อเก็บได้ 11 d แต่ไม่เพิ่มขึ้นhatchability เมื่อเวลาเก็บ 5 d (โต้ตอบ P= 0.0009) เปอร์เซ็นต์การฟักตัว prestorage ที่เพิ่มขึ้นของลูกไก่ชั้นประถมศึกษาปีที่สอง (P = 0.0007) เหมือนที่เก็บเวลา การพัฒนาตัวอ่อนไข่คอลเลคชั่น และผลของกำหนดระยะเวลาฟักตัว prestorageคณะทันตแพทยศาสตร์ prestorage บน hatchability และเจี๊ยบ
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