Anthracnose is caused by the fungus in chili. The spores are mostly spores tapered side. There are 3 species. Found that all three species generated apressorium a light brown - dark. The isolation method tissue transplanting method on PDA then do single spore isolation (SSI), and keep the fungus on the potato. carrot agar (PCA) slant then move onto a plate and PDA fungus food group based on the colony found that the fungus Colletotrichum Classification of species can be divided into C.acutatum, C. capsici and C.gloeosporioides number 88,155,146 isolate, respectively. The area to find C. capsici only a single species. Nakhon Si Thammarat. Part C. gloeosporioides found 100% in Sukhothai for C. acutatum found 90% in Chiang Rai. Morphological studies showed that C. acutatum conidia spores of a single cell size 2.5-5.0 x 2.5-23.75 μm seta not find the creation ofC. Capsici spores have a single cell size of spores 1.25-3.75 x 7.5-32.5 μm seta well for C. gloeosporioides spores 1 cell size of spores 2.5-5.0 x 3.75-23.75 μm seta find building or not building it. This research explores the species of bacteria. Colletotrichum In major growing areas And the study of morphological variation of fungi for the database in the classification of fungi Colletotrichum Anthracnose caused by chili.