Description of Test
The NDV ELISA kit will measure the amount of antibody to NDV in the serum of chickens.
Microtitre plates have been pre-coated with inactivated NDV antigen. Chicken serum samples are
diluted and added to the microtitre wells where any anti-NDV antibodies present will bind and form
an antigen-antibody complex. Non specific antibodies and other serum proteins are then washed
away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and
binds to any chicken anti-NDV antibodies bound to the antigen. After another wash to remove
unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed
if anti-NDV antibody is present and the intensity is related to the amount of anti-NDV antibody
present in the sample.