The media is composed of peptone-10gm, dextrose- 40gm, agar- 20gm for 1000ml of solution. For this study 200ml of media has been prepared, for which the amount of components are peptone 2gm, dextrose- 4gm, agar-2gm. And the pH of the media should be 5.5-6, not greater than 6.