About 1.0000 0.0002 g of C. nutans sample was placed in a
100 mL round-bottom flask and mixed with ethanol (44 %vol). The
system was magnetically stirred to keep it homogenized throughout
the extraction at 40 C. Sampling was done according to the
same schedule used in MAE experiments. The extracts were
filtered through 0.20mm PTFE syringe filters into amber vials that
had been previously swept with nitrogen, and they were stored in
refrigerator (8 C) until further analysis. Extraction experiments
were carried out in triplicate.