In the current study, Sym'Previus tool was used to facilitate the use of recognised mathematical models to describe bacterial behaviour, in particular B. amyloliquefaciens growth in bread yielding ropy bread. As an illustration, two storage scenarios were chosen in this study to account for climate where ropy bread is an issue (storage at 35 C) or not (storage at 18 C). These results confirmed that the bread contamination by B. amyloliquefaciens can be efficiently controlled by using LAB-derived acidifiers as bio- preservatives. Moreover variable growth abilities were observed for the three strains as shown on growth/no growth boundaries (Fig. 1) and on population distributions (Fig. 3) generated by the 10 000 in silico growth simulations. This study highlights a higher rope spoilage potential of the ISPA strains as compared to the ATCC strain which emphasize the interest to characterise both wild strains and reference strains to account for biological variability and the pres- ence of selected or adapted strains with high spoilage potential in ingredients and raw materials.