5. Discussion
Typing of pathogenic bacteria is an important process
in epidemiological studies and control of nosocomial infections.
spa typing and PCR-RFLP in addition to pulsed
field gel electrophoresis (PFGE) are among the efficient
methods for differentiating S. aureus, particularly MRSA
strains (9). In this study, S. aureus isolates were classified
by PCR-RFLP of the spa gene with Bsp143I enzyme, which
classified in seven types.
The spa gene lengths in the isolated cases varied from
1150 to 1500 bp, which were slightly longer than the data
reported from a study in India (1150-1420 bp). The maximum
repetition of the X-region in our samples was 12
times, but the same item in a study from India was 13 (4).