The goals of this study are as follows: (i) to evaluate the magnitude of the classical Osborne wheat
protein fractions (albumins, globulins, gliadins, and glutenins) across the grain of five bread and five
durum wheat genotypes; (ii) to determine different protein components of the four subunits of wheat
gliadin using SDS-PAGE analyses; (iii) to determine the content of tryptophan as an aromatic and
essential amino acid and wet gluten as a quality parameter of wheat flour