Fatty acids are suitable for separation, quantitation and analysis by GC without any derivatization, as demonstrated by James and Martin [34], Lipsky et al. [229], Ackman and Burgher [43], Zoccolillo and Rinchetti [44], and Brondz et al. [45]. Derivatization of fatty acids for GC analysis is performed to increase the volatility of the substances, to improve separation, and to reduce tailing. Recent developments in the technology of columns with bonded phases offer spectacular separation and less phase bleeding.