Fungus are eukaryotic organism and they are classified into two main groups that is yeast and molds.Its cell wall is made up of chitin.Fungal cells have both macroscopic as well as microscopic structure .This micro-organism is useful as well as harmful to human being useful because it produces many antibiotics,natural products as well as used in industrial fermentation process.It is harmful in the sense as it causes human diseases,produce toxic substances as well as harm important crops. So it is very important to study fungal species.The branch of science that deals with study of fungal species is called as Mycology.Staining of these fungal cells is a very important step.These are some examples of fungi Rhizopus, Aspergillus, Penicillium, Fusarium, Candida,Mucor etc.Here we are going to stain fungal cells by using Lactophenol cotton blue method this staining technique is also called as mounting of fungus.