SL-biofilms shown in Figure 4, Additional file 3: Movie 2
and Additional file 4: Movie 3 were established by transferring
the liquid culture (OD 600 nm = 1.0) into Petri
dishes and incubating under static conditions for 7 days.
These mature SL-biofilms were then mechanically homogenized
through repetitive pipetting with a serological
pipette to disrupt the layer of attached biofilm
and large loosely attached aggregates. This was followed
by transfer into a conical tube and gentle vortexing. The
homogenized SL-biofilm culture was then transferred
back into the original culture dish and left to incubate
while photographs were collected.