constant pH value. The concentration of chitin in the final dispersion
was determined gravimetrically to be 1.000% w/v. A transmission
electron microscopy (TEM) analysis of regenerated chitin was
performed using a CM100 TEM (Philips, Holland) at 80 kV. Drops of
diluted chitin suspensions were placed on a carbon-coated copper
grid (400 mesh), dried at room temperature and negatively stained
by uranyl acetate (2%, w/v) for 1 min.