Climatic variables had no apparent effect on HSP70
concentrations in lymphocytes. In the region of the study,
there are two clearly differentiated weather seasons:
warm and cool [32]. In previous studies, we have
observed that, in general, reproductive variables are
significantly impaired during the warmseason [32–34]. It
is well documented that high temperatures induceHSP70
expression in many cells, including lymphocytes [35–
36]. However, in the current study, higher temperatures
during the warm season may not have been sufficiently
severe to significantly increase the concentration of
HSP70 in lymphocytes. The recent introduction of new
cooling systems on this farm may have compensated for
the higher temperatures during the warm period, so that
their effect on HSP70 concentration in peripheral blood
lymphocytes could not be appreciated. In a recent study,
we have observed an improvement in several reproductive
variables during the warm period, which coincided
with the introduction of the new cooling systems,
including fans and water sprinkles [34].