Data in table 1 showed that the meat
soaking with pineapple juice could increase
the tenderness, because of bromelin, which
was protease enzyme, on the juice4. Almost
half of protein content in pineapple was
protease enzyme13. Longer soaking would
make the meat more tender. This showed
that more and more soaking made more
protein from muscle fibers hydrolyzed into
peptide bond, resulting in soft tissue.
According to Wang et al., papain destroyed
sarcolemma and loss collagen capacity,and
become amorphous material21.