2.3. Biosynthesis of silver and gold nanoparticles
For the biosynthesis silver nanoparticles, 1.5 ml of plant extracts
is mixed with 30 ml of AgNO3 solution (1 mM/ml) and incubated at
28 ◦C for 24 h. Small aliquot of solution is used for the UV–Vis spectroscopy
and FTIR is performed to the extract which was exposed
before and after addition to the silver nitrate solution. The reactions
mixture is centrifuged at 6000 rpm for 10 min and the pellet was
resuspended in small amount of sterilized double distilled water
and then small amount of suspension was sprayed on glass slide to
make thin film. The thin film was kept in hot air oven to dry and
then the thin film was used for the SEM analysis equipped with EDS
(Model JEOL, JSM-5610). The same procedure is followed for gold
nanoparticles synthesis