from H. coronarium under consideration, carrageenan-induced paw
edema test was examined according to the method of Winter et al.
(1962). Thirty minutes before application of the inflammatory agent,
the animal groups were orally treated (p.o.) either with 1 ml of
essential oil (100 mg/kg) previously emulsified in 3% Tween 80, or
with 1 ml of indomethacin (10 mg/kg; pos. control) or an equal
volume of 0.9% aq. NaCl (neg. control). Then male Wistar rats were
briefly anesthetized with ethyl ether and injected subplantarly into
the right hind paw with 0.1 mL of 1.0% carrageenan in isotonic
saline. The left hind paw was injected with 0.1 mL of saline to be
used as a control. Measurement of paw size was carried out by
gently wrapping a piece of white cotton thread around the paw and
measuring the circumference on a meter rule (Bamgbose and
Noamesi, 1981; Olajide et al., 2000) at hourly intervals for 5 h after
the stimulus.