Fibers after cell culture were also observed in a Con- focal laser scanning microscope (Olympus, Model: FV500) to detect the extent of cell growth and spread- ing of the cytoskeleton. For the confocal study, the cells were fixed using 4 % paraformaldehyde and then stained with the fluorescent dyes (Phallodin 633 and Hoechst 33342) to stain the cell and nuclei in red and blue, respectively.