For the production of CMCase by A. strictum the best culture condition determined by the Plackett & Burman design was 17 g/L of sugarcane bagasse submitted to mild pretreatment, 2 g/L of molasses, 100 rpm, pH 7.0 and 30 C. For the production of b-glucosidase by A. strictum, the best culture condition was 50 g/ L of the mild sugarcane bagasse, 2 g/L of molasses, 100 rpm, pH 5.0 and 30 C.