We added the venoms alone or the mixtures in
a solution containing0.2%azocasein,20mM CaCl2, and 0.2M Tris–HCl (pH8.8).There action run for 90min at37C and was stopped by the addition of 0.4mL of 15%(vv) trichloracetic acid,and then
centrifuged at 20,000rpm.