The reference extract was obtained by the Randall technique
[18] in the following way. Dry cananga flowers (4 g) were
extracted with 80mL of chloroform heated to boiling point during
2 h using a Velp Scientifica SER 148 extractor. The solution
was then concentrated under a vacuum in a rotary-evaporator at
30 ◦C. Each dried sample was weighed for gravimetric analysis
of extract yield and then diluted with chloroform to 20mL
and stored in the dark at 4 ◦C prior to analysis of oil yield and
composition by GC/MS.
The residual extract was get from the residual flowers (the
flowers after DIC treatment) in the same manner as the reference
extract and analyzed by GC/MS.