Protein quantification
Peak height of peptides belonging to an identified protein was
determined using Bioworks. For the host defense proteins, the 3
most abundant peptides per protein were summed [43]. The same
3 peptides were chosen for the five replicates. The summed
peptide heights were compared between the human and bovine
samples using an independent two-sample t-test, using PASW
statistics 17 (SPSS Inc, Chicago, IL, USA). If a protein was not
detected in a specific sample, the value for the peak height was set
to 104 (minimum detection level) for statistical calculations and
‘‘,1’’ in Table 3.
Acknowledgments