Cell culture and stimulation resulted in classical and non-classical monocyte populations to
combine into one subpopulation of CD14+
monocytes with intermediate expression of CD14
and CD16 compared to baseline expression patterns (Figure 2A). Upon CoPP stimulation, the
CD14+
monocytes expressed higher HO-1 levels compared to their PBS (Figure 2C; p <
0.0001, 2-way ANOVA post-test). Consistent with prior findings [9,11], our observations
suggest that CD14+
monocytes are the most biologically relevant producers of HO-1 in the
peripheral blood.