Based on the data in the suspension tests, the most effective andpractical exposure time for each disinfectant had been selected toapply onto SS chips. Our results demonstrated the significantreduction in the populations ofClostridium perfringesspores aftertreatment with all tested disinfectants (Table 3, Count D) comparedto respective control and AK-induced germination samples(p<0.05). Under dormant condition (shown in the control experiments), only iodophore showed a minor sporicidal activity with0.33e0.42 log reductions when compared with the viable countsfor the untreated spores. The insignificant increase in Count D ofcontrol samples compared with Count A was probably due to theslightly variations of spore inoculum size into each SS chip.Furthermore, after treatment, SS chips became wet with thedisinfectant solution and neutralized water that could facilitatethe removal of adhered spores from the surfaces compared to thedetachment of cells from dry surfaces with wet cotton swabs.Germinating spores were much more susceptible to theantimicrobial effect of disinfectants and resulted in the decreaseof 1.53e2.70 log CFU/chip. Exposing germinated spores with25 ppm iodophore for 5 min being the most effective treatment,which was comparable to treatment with 12.5 ppm iodophore for10 min. Ethanol, Quats and DeconSpore had similar sporicidalefficiency againstC. perfringensspores adhered onto SS surfacesภายใต้เงื่อนไขในการทดลองเหล่านี้ (ตาราง 3)
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