3. Results and discussion
3.1. Optimization of callus induction medium
Vigorous callus growth was observed throughout the entire culture
period. Embryogenic calli quickly developed in all eight induction
media within the first week of incubation. Callus induction rate was
the highest on medium M11AP (84.0%) followed by M11APC1
(78.7%). However, callus fresh weight was significantly lower on
M11AP than on M11APC1 (Table 2). Overall, M11APC1 was found to
be the most efficient callus induction medium for SA281 among the
eight media tested. Similar experiments were preformed to optimize
callus induction media for Tx430 (Fig. 1c) and 91419R (data not
shown). Results indicated that M11APC1 was the optimal medium for
those two lines as well.