Disadvantages
Certain disadvantages are inherent in any optical imaging technique.
By using an aperture diaphragm for contrast, past a certain point, greater contrast adds distortion. However, employing an iris diaphragm will help compensate for this problem.
Brightfield microscopy can’t be used to observe living specimens of bacteria, although when using fixed specimens, bacteria have an optimum viewing magnification of 1000x.
Brightfield microscopy has very low contrast and most cells absolutely have to be stained to be seen; staining may introduce extraneous details into the specimen that should not be present.
Also, the user will need to be knowledgeable in proper staining techniques.
Lastly, this method requires a strong light source for high magnification applications and intense lighting can produce heat that will damage specimens or kill living microorganisms.
Check out many more useful microscopy imaging techniques here.
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