Machine for producing high quality of natural floss was completely designed and developed. From operations research, the researcher additionally informed the optimal conditions for coating natural floss and rolling.These obtained from analysis of user satisfaction and mechanical properties. From testing the cleanliness of productivity, it was shown that natural floss free from microorganisms. For future plan of research, researcher will be find process for adding flavor smells to floss and design an automatic machine for packaging floss into package box.