The plates were spread with 1.5 ml of spore suspension (ca.1 x 106 spores/ml) and incubated at 25 _C for 7 d. Fresh conidia were harvested by flooding the surface of the plates with 4.5 ml of sterile saline solution (NaCl, 9 g/l of water) containing Tween 80 (0.05% vol/vol; Prolabo, Paris, France).